Antibacterial activity of allium sativum against staphylococcus aureus in vitro 347 was 27% at a wave length of 580 nanometer, while the light transmittance was 100% for nutrient broth used to prepare the bacteria jassim, 2003. Studies on the antimicrobial properties and phytochemical screening of garlic allium sativum extracts olusanmi, m. Numerous naturally occurring phytochemicals are present in plant tissues and many studies have evaluated their antimicrobial activities in several plant extracts as garlic allium sativum against bacteria cavallito and bailey, 1994, fungi adetumbi et al. Garlic can provide proper management for bacterial growth ranging from disinfectant, antiseptic, bacteriostatic and even bactericidal characteristics. Louis pasteur was the first described the antibacterial effect of onion and garlic juices. Antimicrobial activity of plant extracts against post. Antimicrobial activity of onion and ginger against two food borne pathogens escherichia coli and staphylococcus aureus volume 1 issue 4 2015 marsunn ortiz hp science and technology centennial college, canada correspondence. Garlic is highly documented in exhibiting powerful antimicrobial activities. Garlic onion aqueous extract showed a compensatory synergistic effect on the three organisms with the zone of inhibition ranging between 924mm. The minimal inhibitory concentration mic of the plant extracts against e. Antioxidant and antimicrobial activities of fresh garlic and. A study of antimicrobial activity of few medicinal herbs. We set out to determine if pure allicin and aqueous garlic extracts age exhibit antimicrobial properties against the burkholderia cepacia complex bcc, the major bacterial phytopathogen for alliums and an intrinsically multiresistant and life. Antimicrobial activity of caraway, garlic and oregano extracts against filamentous moulds gordana r.
Antimicrobial and antioxidant activities of the essential. Antimicrobial effects of calcium oxide nanoparticles and. It has been used as a spice, food and folklore medicine for many years, and has been widely researched as a medicinal plant. Chemical composition of the oil was analysed by gcms and the most representative compounds of the essential oil were organosulfurcontaining compounds, such as methyl 5methylfuryl sulfide 18. Gupta and viswanathan reported that garlic extract stored at room temperature had less activity against b. The inoculated areas of the onion bulbs showed changes in their colour and the tissues macerated then an offensive smell developed. However, little research has been undertaken on the antifungal activity of these extracts. The minimum inhibitory effect of ginger extract was found to be 8. It was found that most plant extracts studied had antibacterial and antifungal activities. The aims of this study were to test the efficacy of essential oil of allium cepa against food spoilage and foodborne pathogenic microorganisms and its antioxidant activity. Ginger extract was effective in restricting the growth of e. Garlic is a hardy perennial member of onion family. Each time 100% garlic juice undiluted was inoculated on nutrient agar media and incubated at 37c overnight and was found to be sterile.
This study investigated the pathogenicity of two fungi a. The antibacterial effect of some medicinal plant extracts and. Studies on the antimicrobial properties and phytochemical. The essential oil eo extracts of these allium plants. Antioxidant and antimicrobial activities of fresh garlic.
Abstract antimicrobial activity of different concentrations 50, 100, 200, 300 and 500 mll of essential oil extracts of three type of onions green, yellow and red and garlic against two bacteria, staphylococcus aureus, salmomella enteritidis, and three fungi, aspergillus niger, penicillium cyclopium and fusarium oxysporum, was investigated. While, onion extracts promote mycelial growth of the fungus p. Antimicrobial activity of essential oil extracts of various onions allium cepa and garlic allium sativum garlic allium sativum l. Garliconion aqueous extract showed a compensatory synergistic effect on the three organisms with the zone of inhibition ranging between 924mm. Alcohol extracts show hypotensive analgesic and antileptazol properties.
Onion allium cepa is a bulbous plant widely cultivated in almost every country of. Onion, garlic, human pathogens, antimicrobial activity, solvent extracts. Antimicrobial activity of different concentrations 50, 100, 200, 300 and 500 mll of essential oil extracts of three type of onions green, yellow and red and garlic against two bacteria, staphylococcus aureus, salmomella enteritidis, and three fungi, aspergillus niger, penicillium cyclopium and fusarium oxysporum, was investigated. Antimicrobial activity of plant extracts against post harvest. The antimicrobial activities of garlic and other plant alliums are primarily based on allicin, a thiosulphinate present in crushed garlic bulbs. Antimicrobial properties of garlic oil against human. Ethanolic extracts thus obtained were immediately evaluated for antibacterial using agar well diffusion method and antifungal activities using poisoned food technique chen et al. Comparatively, 50 and 100 ml l1 concentrations of onions extracts were less inhibitory than 200, 300 and 500 ml l1 concentrations. Apr 14, 2010 antimicrobial and antioxidant activity of crude onion allium cepa, l. Historically, garlic has been used for centuries worldwide by various societies to combat infectious disease.
Antimicrobial and antioxidant activities of edible. The essential oil was extracted by supercritical co 2 extraction in our lab as described by ye and lai 2012. Antimicrobial resistance has been a global concern. Garlic allium sativum has traditional dietetic and medicinal uses as an antiinfective agent 1.
The aim of this study is to test the antibacterial effect of various herbs and spices on escherichia coli. Louis pasteur was the first described the antibacterial effect of onion and garlic juices against both grampositive and gramnegative bacteria. Antifungal studies in the plant extracts of turmeric, ginger. Onion and garlic also known to have medicinal properties, oleoresin of rosemary, can provide inhibition of oxidative rancidity and retard the development of warmedover flavor in some products. Antimicrobial activity of allium cepa against bacteria. Antimicrobial and antioxidant activities of the essential oil. Tanackov and danijela tuco inhibitory effect of caraway, garlic and oregano extracts 0. The antibacterial effect of some medicinal plant extracts. Antimicrobial properties of garlic oil against human enteric.
The inhibitory effect of these spice extracts at varying concentration on listeria monocytogenes is shown in table2. Assessment of antimicrobial activity of onion allium cepa and garlic. Lebensmittelwissenschaft undtechnologiefood science and technology, 37. Jorgensen, map faller, rh yolken manual of clinical microbiology 8th eds. Control of aspergillus niger with garlic, onion and leek. Agar well diffusion method the antibacterial activity of two crude ethanolic extracts of clove and garlic plant parts against six food. A significant growth inhibition was shown by most of the organisms, tested at random. Antimicrobial activity of the extract of garlic and onions. Benkeblia n, antimicrobial activity of essential oil extracts of various onions a. The screening of antibacterial activity of extracts was carried out using the agar well.
Pdf antibacterial activity of garlic extract allium sativum against. The antimicrobial activity of garlic is due to the sulfur organic compounds mainly allicin. Antibacterial activity of garlic extract against human pathogenic. Allium sativum garlic a natural antibiotic pdf free. The antibacterial activities of extracts were evaluated using the disk diffusion method as well as well diffusion method. Garlic and onion are widely used for flavoring of foods. A classroom demonstration of garlic extract and conventional. Therefore, the methanolic extracts from different parts of four medicinal plants used locally in folk medicine were evaluated for antimicrobial activity.
Antioxidant properties, antimicrobial activity, natural additive, garlic peel extract 1. Antimicrobial and antioxidant activity of crude onion allium cepa, l. The onion, a natural alternative to artificial preservatives. Antimicrobial and antioxidant activities of red onion, garlic. However, little information is available emphasizing the preservative and antimicrobial properties of spices.
Food science and biotechnology 2011, 20 4, 11011106. This study was conducted to elucidate minced beef stabilization properties of hydroalcoholic extracts of commonly used culinary spices from pakistan against meat oxidative stress and microbial spoilage. The essential oil revealed an interesting antimicrobial effect against the tested microorganisms with the mic and mbc values in the ranges of 0. Polyphenolrich extracts of traditional culinary spices and. Leek extracts showed positive antioxidant activities and positive antimicrobial activities kyounghee et al. Antibacterial effect of herbs and spices extract on. Aqueous extracts of garlic allium sativum and onion allium cepa were tested for activity against grampositive. Antimicrobial activity of essential oil extracts of various. Antimicrobial activities of garlic and ginger were carried out using the following microorganisms. In addition to its nutritional effects, the antioxidant and antimicrobial activities of onion has also.
Antimicrobial activity against staphylococcus aureus from methanolic and aqueous extracts of dried a. To evaluate the antimicrobial activity of fresh juice of. There are higher quantities of sulphur compounds allicin in garlic than onion. In vitro confirmation of the antimicrobial action of fresh and freezedried garlic extracts against human pathogenic bacteria 2, fungi 3, and viruses 4 supports these applications. Test methodologies were identified as capable of producing underestimates of go activity. Investigation of antioxidant and antimicrobial properties of. Antibacterial activity of extracts of allium sativum garlic and allium cepa. Its close relatives include the onion, shallot and leek. Antimicrobial and antioxidant activities of edible coatings. Moreover, these spices contain antimicrobial compounds. Both ginger and garlic extracts have bacteriostatic effect on the organism. Phytochemicals present in medicinal plants have health benefits and antimicrobial activity against some pathogenic bacteria. The antibacterial effect of ginger and garlic extracts on. Herbs and spices are very important and useful as therapeutic agent against many pathological infections.
Antimicrobial activity and thiosulfinates profile of a. Antibacterial effects of ag were higher than those of ng. The pces of these allium plants garlic and onions exhibited marked antibacterial activity, with garlic showing the highest inhibition and green onion the lowest. All extracts showed a wide range of total phenolic. The plant extract ethanolic, essential oils and aqueous were prepared from onion, garlic and leek at. Marsunn ortiz, hp science and technology centennial college, 755 morningside ave, scarborough, on m1c, canada, tel 647. In vitro antibacterial activity and stability of garlic. Antimicrobial activity of ethanolic extracts of syzygium. The study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion allium cepa, garlic allium sativum, mint mentha spicata, thyme thymus vulgaris, oak quercus, aloe vera aloe barbadensis miller, and ginger zingiber officinale. Antimicrobial activity of essential oil extracts of.
It has been reported that a human would need to eat. Garlic peel ethanol extract demonstrated both antioxidant and antibacterial activities in cooked beef. Antimicrobial activity of phenolic compound extracts of. Evaluation of phenolic compounds and antioxidant and. Elnima ei, ahmed sa, mekkawi ag, mossa js, the antimicrobial activity of garlic and onion extracts. Besides that, garlic may have the ability to prevent and manage viral, fungal and even helminthes infections. In the present study the antimicrobial potency of garlic and ginger has been investigated against eight local clinical bacterial isolates. The antibacterial effects of each onion water extraction against these 6 bacteria species were carried out in vitro by. Preparaton of standard dilutions of garlic extract the dilution were prepared by using ethylene glycol which. Antibacterial and antioxidant activities of quercetin. Department of plant biology, university of ilorin, pmb 1515, ilorin, nigeria issued april 1, 2010 abstract this study evaluates the antimicrobial properties of garlic allium sativum extracts on three fungi. Inhibitory effect of allium sativum and zingiber officinale. Control of aspergillus niger with garlic, onion and leek extracts. Ali regional center for food and feed rcff, agricultural research center, giza, egypt.
Antimicrobial and antioxidant activities of red onion. Antimicrobial properties of spices have been documented in ancient literature and the interest continues to the present 1114. Apr 27, 2012 herbs and spices are very important and useful as therapeutic agent against many pathological infections. Introduction allium is a monocot genus of flowering plants, informally referred to as the onion genus. Antimicrobial solutions assayed indicator microorganisms casein solution native micro. Antimicrobial and antioxidant activities of red onion, garlic and leek. Garlic can be provided in the form of capsules and powders, as dietary supplements, and thus differ from conventional foods or food ingredients.
When the organism was at exponential phase of growth, extracts of ginger and garlic were made using decoction method. Among the onion and garlic, garlic is found to be slightly better species in antimicrobial activity than onion. Methodologies for estimating the antimicrobial activity of go were assessed and go, go sulfide constituents, and garlic powder gp were compared in tests against human enteric bacteria. This filtrate was considered 100% fresh garlic extract, was stored at. Introduction garlic is widely used around the world for its pungent flavor as a seasoning or condiment. Garlic extracts reported activities include reducing cholesterol, tumor suppressing, and acting as an antifungal, antibacterial, antiviral and antiparasitic agent reuter et al. All extracts showed a wide range of total phenolic contents, that is, 4. This research aim at testing the antifungal activity of organic ethanol extracts of onion allium cepa, ginger zingiber officinale and garlic allium sativum against three. Investigation of antioxidant and antimicrobial properties. Antibacterial activity of aqueous garlic allium sativum. The development of new antimicrobial agents against resistant pathogens is increasing interest. This study evaluates the antioxidant and antimicrobial activity of spice extracts such as ginger, turmeric and garlic by 2, 2diphenyl1picrylhydrazyl dpph radical scavenging method and also to evaluate their antimicrobial effects by slant method. Research article open access antimicrobial activity of.
Antimicrobial and antioxidant activities of red onion, garlic and leek in sausage abdelsalam, a. In this paper we evaluated the antimicrobial activity of crude extracts of garlic against seventeen bacterial pathogens and also an attempt has been made to study the effect of temperature and ph on the stability of the extracts against two gram positive and two gram negative pathogens. Research article open access antimicrobial activity of onion. Test methodologies were identified as capable of producing underestimates of go. Garlic extract at the higher concentration completely inhibited radial growth of both fungi. Polyphenolrich extracts of traditional culinary spices. A comparison of the antifungal properties of onion allium.
Chemical composition, antibacterial and antioxidant. Currently, interest has been focused on exploring antimicrobial properties of plants and herbs. Garlic garlic allium sativum is among the oldest of all cultivated medicinal plants. Antifungal studies in the plant extracts of turmeric. Introduction garlic is widely used around the world for its pungent flavor as a. Onion allium cepa is among the oldest cultivated plants, and is used for multiple purposes. Essential oil extracts of onion and garlic can be used as natural antimicrobial additives incorporated in various food products 1617. The antimicrobial effects of aqueous garlic extracts are well established but those of garlic oil go are little known. Pdf antimicrobial activity of the extract of garlic and. The antimicrobial activity of garlic and onion extracts.
Increasing multidrug resistance of pathogens forces to find alternative compounds for treatment of infectious diseases. In conclusion, aged garlic showed more potent antioxidant and antibacterial effects than fresh garlic. Pdf antimicrobial activity of the extract of garlic and onions. Hydroalcoholic extracts of six selected spices, namely, onion, ginger, turmeric, coriander, fennel, and mint, were evaluated to inhibit microbial growth in minced beef under refrigerated. Chitosan solution carboxymethyl cellulose solutions oleoresins at 1% concentration. Aqueous extracts of garlic allium sativum and onion allium cepa were tested for activity against grampositive organisms, gramnegative organisms and fungi.
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